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rabbit anti ppp2r1a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ppp2r1a
    Rabbit Anti Ppp2r1a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ppp2r1a/PP2A+A+Subunit+Rabbit+mAb/pmc12350166-357-23-25
    Average 93 stars, based on 121 article reviews
    rabbit anti ppp2r1a - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy.
    Article Snippet: The sgRNA was cloned into pLentiCRISPR V2 (Addgene, 83480).

    Immunofluorescence:

    Article Title: The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.
    Article Snippet: D ow nade The primary cilium is required for Hedgehog (Hh) signaling in vertebrates.. Hh leads to ciliary accumulation and activation of the transmembrane protein Smoothened (Smo) and affects the localization of several pathway components, including the Gli family of transcriptional regulators, within different regions of primary cilia.. Genetic analysis indicates that the kinesin protein Kif7 both promotes and inhibits mouse Hh signaling.

    Control:

    Article Title: The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.
    Article Snippet: D ow nade The primary cilium is required for Hedgehog (Hh) signaling in vertebrates.. Hh leads to ciliary accumulation and activation of the transmembrane protein Smoothened (Smo) and affects the localization of several pathway components, including the Gli family of transcriptional regulators, within different regions of primary cilia.. Genetic analysis indicates that the kinesin protein Kif7 both promotes and inhibits mouse Hh signaling.

    Western Blot:

    Article Title: The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.
    Article Snippet: D ow nade The primary cilium is required for Hedgehog (Hh) signaling in vertebrates.. Hh leads to ciliary accumulation and activation of the transmembrane protein Smoothened (Smo) and affects the localization of several pathway components, including the Gli family of transcriptional regulators, within different regions of primary cilia.. Genetic analysis indicates that the kinesin protein Kif7 both promotes and inhibits mouse Hh signaling.



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    Validation of interactions between the OmpP2 protein and host cell proteins based on co-IP analysis. (A) Western blot analysis of the expression of Flag-CAV1, Flag-ARF6, and <t>Flag-PPP2R1A</t> plasmids transfected into HEK 293T cells and Pet-28a-HA-His-OmpP2 transformed into E. coli BL21(DE3). (B) Immunoblot of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and HA-OmpP2 protein using anti-HA MAb. (C) Immunoblot of HA-OmpP2 protein and host cell proteins precipitated using anti-Flag MAb from HEK 293T cells transfected with pCAGGS-Flag-CAV1, pCAGGS-Flag-ARF6, or pCAGGS-Flag-PPP2R1A.
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    Validation of interactions between the OmpP2 protein and host cell proteins based on co-IP analysis. (A) Western blot analysis of the expression of Flag-CAV1, Flag-ARF6, and <t>Flag-PPP2R1A</t> plasmids transfected into HEK 293T cells and Pet-28a-HA-His-OmpP2 transformed into E. coli BL21(DE3). (B) Immunoblot of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and HA-OmpP2 protein using anti-HA MAb. (C) Immunoblot of HA-OmpP2 protein and host cell proteins precipitated using anti-Flag MAb from HEK 293T cells transfected with pCAGGS-Flag-CAV1, pCAGGS-Flag-ARF6, or pCAGGS-Flag-PPP2R1A.
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    Image Search Results


    Validation of interactions between the OmpP2 protein and host cell proteins based on co-IP analysis. (A) Western blot analysis of the expression of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A plasmids transfected into HEK 293T cells and Pet-28a-HA-His-OmpP2 transformed into E. coli BL21(DE3). (B) Immunoblot of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and HA-OmpP2 protein using anti-HA MAb. (C) Immunoblot of HA-OmpP2 protein and host cell proteins precipitated using anti-Flag MAb from HEK 293T cells transfected with pCAGGS-Flag-CAV1, pCAGGS-Flag-ARF6, or pCAGGS-Flag-PPP2R1A.

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Validation of interactions between the OmpP2 protein and host cell proteins based on co-IP analysis. (A) Western blot analysis of the expression of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A plasmids transfected into HEK 293T cells and Pet-28a-HA-His-OmpP2 transformed into E. coli BL21(DE3). (B) Immunoblot of Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and HA-OmpP2 protein using anti-HA MAb. (C) Immunoblot of HA-OmpP2 protein and host cell proteins precipitated using anti-Flag MAb from HEK 293T cells transfected with pCAGGS-Flag-CAV1, pCAGGS-Flag-ARF6, or pCAGGS-Flag-PPP2R1A.

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Biomarker Discovery, Co-Immunoprecipitation Assay, Western Blot, Expressing, Transfection, Transformation Assay, Recombinant

    Overexpression of CAV1, ARF6, and PPP2R1A promoted the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) Transfection of the expression plasmids and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001. (C) Phagocytosis of G. parasuis by transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Overexpression of CAV1, ARF6, and PPP2R1A promoted the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) Transfection of the expression plasmids and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001. (C) Phagocytosis of G. parasuis by transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Over Expression, Transfection, Expressing, Western Blot

    Knockdown of CAV1, ARF6, and PPP2R1A impairs the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) siRNA silencing and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. **, P < 0.01; ***, P < 0.001. (C) Phagocytosis ability of G. parasuis by iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Knockdown of CAV1, ARF6, and PPP2R1A impairs the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) siRNA silencing and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. **, P < 0.01; ***, P < 0.001. (C) Phagocytosis ability of G. parasuis by iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Knockdown, Western Blot

    Several domains of OmpP2 are sufficient for CAV1, ARF6, and PPP2R1A interactions. (A) OmpP2 protein structural representation for the standard reference strain G. parasuis serotype 5. (B) Schematic of the OmpP2 domain architecture and constructs used in domain mapping studies. (C) Immunoblot using anti-Flag MAb for Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and mutant proteins.

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Several domains of OmpP2 are sufficient for CAV1, ARF6, and PPP2R1A interactions. (A) OmpP2 protein structural representation for the standard reference strain G. parasuis serotype 5. (B) Schematic of the OmpP2 domain architecture and constructs used in domain mapping studies. (C) Immunoblot using anti-Flag MAb for Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and mutant proteins.

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Construct, Western Blot, Recombinant, Transfection, Mutagenesis

    Primers used for plasmid construction

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Primers used for plasmid construction

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Plasmid Preparation, Sequencing

    siRNA sequences used in this study

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: siRNA sequences used in this study

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: Sequencing, Negative Control

    Antibodies used in this study

    Journal: Microbiology Spectrum

    Article Title: TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

    doi: 10.1128/spectrum.02307-22

    Figure Lengend Snippet: Antibodies used in this study

    Article Snippet: PPP2R1A , PPP2R1A rabbit polyclonal Ab , Proteintech , 15882-1-AP.

    Techniques: